History of Chicken mAbs

While mice are the animal of choice for making monoclonal antibodies and the rabbit is typically used for making polyclonal antibodies, during the past thirty years some mammalian antigens fail to induce an immune response in either rodents or rabbits especially in the case of highly conserved proteins. In cases where an immune response was lacking in immunized mice or rabbits, investigators have turned to the chicken, particularly when polyclonal antibody preparation fails to meet the experimental objectives.

Additionally, one of the reasons chickens have not gained popularity as an immunization animal is the lack of robust technology for making chicken monoclonal antibodies. The hybridoma technology for producing monoclonal antibodies was developed for mice by G. Kohler and C. Milstein in 1975. In 1995, Katherine Knight and her colleagues, at Loyola

University of Chicago, succeeded in developing a rabbit hybridoma technology for developing the rabbit monoclonal antibody. Until recent years, Phage Display technology has been utilized in the field and made chicken monoclonal antibody possible.



Fifty-million-year-old polymorphism at an immunoglobulin variable region gene locus in the rabbit evolutionary lineage Proc. Natl. Acad. Sci. USA 1999


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