Antibody isotype switching (or class switching) is a biological feature of the humoral immune response, in which a switch from IgM to other Ig follows first exposure to an antigen during the immunization. During this process, the constant region portion of the antibody heavy chain is replaced with a different chain, but the variable region of the heavy chain stays the same. Therefore, isotype switching does not affecting antigen specificity. Instead, the antibody retains affinity for the same antigens.
Antibody Isotype Switching
By antibody engineering techniques, we provide services to switch among the five main immunoglobulin classes (IgG, IgA, IgD, IgE, and IgM), mostly IgG1 has been used for engineering therapeutic antibodies. More recently IgG2, IgG4 as well as engineered hybrids have been used to adjust the recombinant molecules better to the targeted application (e.g. regarding half-life, effector functions).