Stable Cell Pool
When the yield of transient expression is not enough and stable cell line development take too long, there is a choice of Stable Cell Pool development. Stable cell pool development takes about 4 weeks. The pool can be bulked to a hundred liters without loss the yield.
1. Once transfected, cells are allowed to grow for three weeks as a pool under selection pressure. As pool bulking up, preserve some vials for multiple lot of expression is possible. Large scale of 10-liter Wave bioreactor production is reasonable for cell pool production.
2. As an option, cells that have been stably transformed can be sorted using the specific antibody against target protein. This technology provides a selection of high yield cell pool.
3. The established stable cell pool is suitable for studying gene function, expression, toxicity, proliferation, as well as for small to medium scale production of recombinant proteins.
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