Induction Conditions
Protein expression is induced by the addition of the proper inducer or by changing the growth conditions. From this point on the cells will use most of their resources for the production of the target protein and will not grow much further.
- The most commonly used promoters induction conditions are listed below.
| Promoter | induction | typical condition | range |
|---|---|---|---|
| trc (hybrid) | IPTG | 0.2 mM | 0.05 - 2.0 mM |
| araBAD | l-arabinose | 0.2% | 0.002 - 0.4 % |
| T7-lac op | IPTG | 0.2 mM | 0.05 -2.0 mM |
- After induction the cultures are incubated from 3 hours to overnight depending on the induction temperature.
| Incubation temperature | incubation time |
|---|---|
| 18°C | overnight |
| 30°C | 5-6 h |
| 37°C | 3-4 h |
