Protein Expression Services

EXONBIO provides molecular biology services including:

• Gene Cloning

  Clone a gene from genomic DNA, cDNA library or BAC and clone into your vector. We also provide subcloning services from cloning vectors to expression or specialized customer vectors.

• Gene Synthesis

  When genes are hard to clone or there is no template available, we can synthesize the gene of interest.

• Mutagenesis

  We provide any kind of mutagenesis such as point mutation, deletion and insert. We can also perform mutagenesis with plasmids as large as 15 kb.

• Antibody Sequencing

  Cloning and sequencing of leader sequence and variable region of antibody heavy and light chains from a hybridoma cell line.

• cDNA Library Construction and Screening

  Construction of cDNA libraries from as little as 200 ng mRNA. cDNA is directionally cloned into an expression plasmid without being digested by restriction enzymes.

• Antibody Services

  • Monoclonal Antibody Development
  • • Rabbit mAb development
    • Chicken mAb development
    • Llama mAb development
  • Antibody Production
  • Antibody Engineering
  • • Isotype Switching
    • Antibody Affinity Maturation
    • Antibody Humanization
  • Hybridoma Sequencing

• Protein Services

  • Mammalian Expression System
  • • Transient Expression
    • Stable Cell Pool
    • Stable Cell Line Development
  • Bacterial Expression
  • Peptide Synthesis
  • Molecular Biology
  • • Gene Synthesis
    • Gene Cloning
    • Site Directed Mutagenesis

• Particle Services

  • Magnetic Particles
  • Particle Size Standards