Description:

1. Bone morphogenetic protein 2 (BMP2) is a member of the BMP subgroup of the TGFβ superfamily. It plays a dominant role in embryonic dorsalventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region. Proteolytic removal of the propeptide enables mature BMP2 to form active disulfide linked homodimers and heterodimers with BMP7. Mature monomeric BMP2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure. Mature human BMP2 shares 100% aa sequence identity with mouse and rat BMP2. It shares 85% aa sequence identity with human BMP4 and less than 51% with other BMPs. BMP 2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPRIA, or BMPRIB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair. It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells.

Source:

1. Chinese Hamster Ovary cell (CHO)

Specific Activity:

1. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.
2. The ED50 for this effect is typically 40-100 ng/mL.

Source:

1. Chinese Hamster Ovary cell (CHO)

Predicted Molecular Mass:

1. 16 kDa, reducing conditions

Specific Activity:

1. The ED50 for this effect is typically 40-100 ng/mL.


2. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.

Endotoxin Level:

1. < 1.0 EU per 1 μg of the protein by the LAL method.

Purity:

1. >95%, by SDS-PAGE under reducing conditions and visualized by silver staining.

Formulation:

1. Lyophilized from a 0.2 μm filtered solution in Glycine, Sucrose, Tween® 80 and Glutamic Acid with BSA as a carrier protein. See Certificate of Analysis for details.

Technique Documents

1. Technique Documents