Commonly Used Tags
Tag | Size | Type | Comments |
---|---|---|---|
His-tag | 6, 8, or 10 aa | Purification | Both native or denature purification. |
GST | 26 kD | Purification | Native purification. Increase solubility. |
Maltose binding Protein | 40 kD | Purification and secretion | Amylose affinity purification |
Strep II | 8 aa | Purification | |
FLAG | 8 aa | Purification and detection | Antibody purification, expensive. |
Protein A | 14 kD | Purification | Methotrexate-agrose purification. |
DHFR | 25 kD | Purification | Methotrexate-agrose purification. |
Cellulose binding Domain | 107 aa | Purification and secretion | Cellulose based resin for affinity purification. |
Calmodulin binding protein | 4 kD | Purification and detection | Calmodulin/Ca2+ affinity purification. |
HA | 8 aa | Purification and detection | Antibody purification, expensive. |
c-myc | 22 aa | purification | antibody purification, expensive. |
NusA | 55 kD | Enhance solubility | Potentially improve solubility. |
Ubiquitin | 76 kD | Enhance solubility | Potentially improve solubility |
GFP | 25 kD | Detection | Used as a report gene. |
HSV | 11 aa | Purification | monoclonal antibody-based purification, denaturing low pH elution needed. |