Your Outsourcing Partner for Protein Research and Production Services

To meet the needs of our clients, Exon provides protein characterization of our expressed protein to insure the quality of recombinant protein.

Recombinant Protein Expression

Exon BioSystems is a protein contract research and production services company specializing in recombinant protein expression, protein purification, and protein characterization.

Antibody Engineering

Additional areas of expertise include assay development of G-protein coupled receptor, recombinant vaccine development and recombinant antibody production.

Biologics

Exon BioSystems is a protein contract research and production services company specializing in recombinant protein expression, protein purification, and protein characterization.

Molecular Biology Services

Gene Synthesis


If your gene of interest is rare in nature, or the genetic material containing your gene is difficult to obtain or isolate, we can instead synthesize your gene of interest de novo.

Gene cloning always requires a gene template. What if you do not have the template of the gene you want? Gene synthesis can help you. With a virtual sequence from gene bank, we can synthesis the gene you need. Gene synthesis is even more cost-effective than gene cloning.

  • Gene Size: no limit.
  • Gene complexity: High GC ratio, highly repetitive sequence and long polyA tails.
  • Turn Around: fastest gene synthesis.
  • Free Codon Optimization: if requested.
  • Quality: Synthesized genes will be completely sequenced to make sure that the synthesized genes perfectly match your requirement.
  • Flexibility: Synthesized gene will be cloned into a pCR-Blunt or pUC19 vector or customer provided vectors.
  • Deliverable: 2 μg of plasmid and a glycerol stab of DH5a strain harboring your plasmid with data sheets including sequencing chromatograms and sequence analysis.
  • Low Cost: see gene synthesis service.
  • More plasmid DNA required? Plasmid prep is available.
  • Customer vector required? Subcloning to customer vector is available.
  • Need to modify your vector? Vector construction and modification.
  • Expression profiling? Recombinant protein expression.
  • Large scale protein expression and purification? Recombinant protein expression.

  • To replace gene cloning.

The technology development in the last a few years made gene synthesis more cost-effective than gene cloning. When the RNAs in nature are rare or not available, gene synthesis is the only choice. Even if RNAs are abundant in tissue, the cost of gene cloning, which includes sample collection, RNA extraction (or DNA extraction if no intron in a specific gene), cDNA construction (or if you buy a cDNA pool), PCR amplification and cloning, as well as mutation detection and correction. The time and cost is usually higher than you anticipated. With gene synthesis at price less than $1 a base, using our gene synthesis service will save you more money, no mention the speed of the gene synthesis and the flexibility of gene optimization.

  • To clone genes without template.

Along with the development of genome sequencing, more and more of the genomes of diverse organisms had been posted on database. Recently the large scale sequencing allows sequencing genomes of mixture of genomes from samples, such as soil or water, without separation or culture of the microbes. Even with common microbes, not all of them are accessible to a regular lab, gene synthesis give you the freedom to clone any gene in database.

  • To improve protein expression

To efficiently express a protein heterogeneously requires modification of the codon usage of the target gene. Numerous published examples has shown codon optimized genes generate more proteins than original genes. It is worthy to optimize your gene before you express it in a heterogeneous expression system. Efficient expression of your target protein saves you time and cost by reducing the amount of culture you required and also easing your downstream purification, therefore, increasing the quality of the protein.

  • To synthesize gene variants.

When you make many variants for a target gene such as an antibody library, gene synthesis is a way to go. Unlike site-directed mutagenesis, multiple mutations (any number of mutations, deletion, insertion or point mutations) can be synthesized in one gene in a single process. By gene variant synthesis, you save your time from days to even months.

  • To make mutation libraries.

Similar to gene variant synthesis, mutation library synthesis is another example of the power of gene synthesis. By making degenerated oligos, we can synthesize completely saturated multi-point mutations in days.

  • To freely design and make new vectors or complex constructs.

With the tool of gene design and synthesis, any virtual DNA sequence can be synthesized on our platform. You have the freedom of designing new gene that can be modified andcloned into any constructs.

ExonBio has developed a high-efficient, cost-effective and robust system to synthesize genes de novo to meet the increasing needs of the broad scientific community and biotechnology industry. The novel platform consists of three modules, enhanced gene optimization and robust oligo assembling, as well as error prevention and error correction.

With the enhanced gene optimization module, protein expression of the optimized gene will be dramatically increased comparing to non-optimized original genes. Our new assembling module enhanced its high throughput and made gene assembling more robust than ever. This, genes with high GC ratio, genes with highly repetitive sequences, and genes with polynucleotides, such as PolyA tail, can be successfully synthesized with shortest time in a high-efficient way.

Our error prevention and reduction module made our gene synthesis service fast and cost-effective. ExonBio provided a better choice for your custom gene synthesis.

From our experiences, more than half of our customers synthesize the gene for protein expression; therefore, codon optimization is the single most important factor in prokaryotc protein translation. The frequencies with which different codons are used vary significantly among different organisms, between abundant and rare proteins within the same organism. The codon optimized sequence perfectly fits the abundances of cognate tRNAs within the cell. Since a synthetic gene is designed as you wish, the codons used to encode different amino acids can be chosen to suit the expression host. There are numerous codon optimization algorisms available online. We have our gene algorithm to integrated codon optimization with respect to GC ratio, secondary structure and mRNA stability to maximize the translation efficiency.

Enhance heterologous protein expression

Optimize codon usage with the cell organism to be used to express the protein Remove secondary structure and RNA unstable sequences. Adjust GC content.,

Modify your gene to suit your needs:
  • Freely add or remove of restriction sites to facilitate gene manipulations.
  • Add N- or C-terminal tags for affinity purification, conjugation or modification.
  • Add linkers. ribosome binding sites, optimal Kozak consensus sequence, leading sequence , and introns.
  • Freely add or remove any site for protein modifications, such as: phosphorylation sites, glycosylation sites, PEGylation sites.
ExonBio provides gene optimization at no extra charge.

Full Service:

Provide complete services from virtual sequences to physical clones, including codon optimization, oligo design and synthesis, gene assembling, cloning, error correction and complete sequence confirmation.

Price: $0.85/base

Quality: synthesized gene is cloned and fully sequenced.

Standard turn-around time
Gene Size Turnaround (days)
<1 kb 14
1~2 kb 16
2~3 kb 20
Deliverable:

ExonBio has developed a high-efficient, cost-effective and robust system to synthesize genes de novo to meet the increasing needs of the broad scientific community and biotechnology industry. The novel platform consists of three modules, enhanced gene optimization and robust oligo assembling, as well as error prevention and error correction.

  • At least 2 ug of plasmid DNA. (more DNA is available if requested)
  • Agar stock of DH5a cells encompassing your plasmid DNA. (glycerol stock is available)
  • Electronic copy of chromatogram files of sequencing data and data analysis.

Express Service:

Our gene synthesis Express Service provides the fastest service in the field. 5 days for genes less than 1 kb.

Price: $1.45/base

Standard turn-around time
Gene Size Express Standard
<500 bp 5 14
0.5~1 kb 6 14
1~2 kb 8 16
2~3 kb 10 20
>3 kb Upon Request
Deliverable:
  • At least 2 ug of plasmid DNA. more DNA is available if requested.
  • Agar stock of your plasmid DNA in DH5a cells. glycerol stock is available.
  • Electronic copy of chromatogram files of sequencing data and data analysis.

Error-free Fragment Service:

To meet the academic need, we provide the fragment service by delivering high quality error-free synthetic gene without cloning and sequence confirmation.

Quality:
  • Fragment ready to clone. The fragment we delivered is assembled, amplified, gel purified and read to clone.
  • High confidence error-free.

By developing a complete new protocol, we have the 95% confidence of hit a perfect gene if 5 clones are sequenced and your gene is less than 1 kb in size. This outstanding accuracy is achieved by precautious in every step during the gene synthesis. More information please visit gene synthesis technology .

Quality of synthesized error-free fragment
Gene Size Error Rate # of clones suggested to sequence
<500 bp 1/2000 3
0.5~1 kb 1/2000 5
1~2 kb 1/2000 8
>2 kb Not suggested
Turnaround:
Turn around time table of error-free fragment
Gene Size Turnaround (days)
<500 bp 5
0.5~1 kb 6
1~2 kb 7
Price: $0.59/base

For more information please call _858-457-1920 or e-mail us at: info @ exonbio.com, or use our contact page: Contact