Anti-idiotype Antibody Development Service
Exonbio’s Idiotypic antibody discovery with SPIN® technology is a leading platform for the delivery of anti-idiotypic antibody reagents. With over 15 years of experience and hundreds of anti-idotypic antibody development projects, our SPIN® technology is a perfect fit for diverse assay applications like PK/PD studies, ADA, and more with high efficiency and rapid timelines.
Benefits
Powerful dual selection
Powerful dual selection makes anti-idiotype antibody development extremely efficient. Positive selection with your target antibody and counter selection with isotype control
Broad spectrum of diversity
Develop anti-idiotype antibodies with neutralization, blocking activities or antibodies for therapeutic antibody detection at once
Human antibody
Convenient human antibody for immunization and screening. No Fab and scFv preparation is required. No papain or pepsin digestion needed.
Benefits
Powerful dual selection
Powerful dual selection makes anti-idiotype antibody development extremely efficient. Positive selection with your target antibody and counter selection with isotype control
Broad spectrum of diversity
Develop anti-idiotype antibodies with neutralization, blocking activities or antibodies for therapeutic antibody detection at once
Human antibody
Convenient human antibody for immunization and screening. No Fab and scFv preparation is required. No papain or pepsin digestion needed.
Anti-Idiotype antibody development with SPIN® tech
Anti-idiotype antibody development is an essential step for therapeutic antibody drug development. Anti-idiotype antibody developed using SPIN® technology can identify target specific plasma cells with dual selection, a positive selection with target antibody and a counter selection with isotype control IgG on FACS sorter. Single Plasma Cell Interrogation (SPIN®) technology applies plasma cell identification and isolation technology, as well as single cell antibody gene amplification, which significantly increases the efficiency to generate anti-idiotypic antibody on a regular and successful basis. The high efficiency of SPIN® technology generates large numbers of anti-ID antibody clones for screening, making it easy to isolate blocking and non-blocking anti-idiotype antibodies in a single run for PK/PD studies.
Figure 1. Illustration of an antibody structure highlighting the variable regions.
Service Highlights
Proven track record of 100% success rate
Identify antibody pairs by epitope binning
Affinity ranking using Biacore kinetic analysis
Royalty free antibodies
Anti-Idiotype Project
Anti-idiotype antibody developed using SPIN® technology is capable of identifying target-specific plasma cells with dual selection, a positive selection with target antibody, and a counter-selection with isotype control IgG on FACS sorter.
An example of an anti-idiotype antibody developed using SPIN® technology. The target antibody drug is a human IgG4 kappa antibody, with isotype control IgG4 for counter selection on FACS sorter.
Table 1: 96-deep well plate expressed supernatants were assayed with indirect ELISA. Upper panel: ELISA plate coated with target humanized target antibody and detected with HRP conjugated secondary anti-rabbit IgG Fc. Bottom panel: ELISA plate coated with corresponding isotype control.