IVD Antibody Development Service
Exonbio provides custom IVD antibody development service using the SPIN® technology that has outperformed other traditional methods by increasing the specificity and sensitivity by 10 folds.
Benefits
Rabbit monoclonal is superior to mouse monoclonal
Rabbit monoclonals are 10 times higher affinity as compared to mouse monoclonals more reproducible as compared to hybridoma and culturing
Antibody pairing
SPIN® technology analyzes native paired antibody genes
Recombinant antibody has better consistency
With antibody sequence in hand, recombinantly developed antibodies ensures the clonality and lot-to-lot consistent
High specificity
SPIN® technology’s counter selection eliminates cross-reactivity
Benefits
Rabbit monoclonal is superior to mouse monoclonal
Rabbit monoclonals are 10 times higher affinity as compared to mouse monoclonals more reproducible as compared to hybridoma and culturing
Recombinant antibody has better consistency
Recombinantly developed antibodies ensures preservation of genetic information.
Antibody pairing
Identify matched antibody pairs by epitope binning
High specificity
Antibodies generated using SPIN® technology minimizes background and eliminates cross-reactivity
SPIN® technology for better IVD antibodies
Antibodies are a vital reagent for sensitive immunoassays. SPIN®, proprietary platform uses microfluidic technology to recognize single plasma cells and isolate only the antigen specific cells. By combining the power of rabbit’s unique immune system and SPIN® technology’s efficient isolation of antigen specific plasma cells generates large number of ELISA positive high affinity antibodies. The large number of ELISA positive clones can be screened for desired binding specificity and kinetics suited for intended immunoassays.
Secondary screening to select the best IVD antibody
1. Kinetics screening with Biacore 8K – Antibody ranking with Ka and Kd
2. Epitope binning – Find a good-matched antibody pairs
3. Flow cytometry screening
4. Immunohistochemistry (IHC)
Screening Data
Highly specific antibodies from plasma cells are generated against nucleocapsid and spike protein of SARS-CoV-2 using SPIN® technology. The recombinant antibodies developed have no cross reactivity against human coronavirus nucleocapsid protein.
Epitope Mapping
High-affinity monoclonal antibodies that fall in different epitope bins allow for antibody pairing for the development of ELISA or lateral kit.
Network plot showing relationship between different monoclonal antibodies by a cord connecting these antibodies.
Neutralization Assay
The two clones S185 and S422 display the best neutralization against the SARS-CoV-2 pseudo virus.The neutralization IC50 values are recorded in micro grams per millimeter.
Neutralization assay Anti- RBD clones S185 and S422 were assessed for neutralization activity on VSV pseudotyped virus carrying a spike protein of SARS,MERS, SARS-CoV and SARS-COV-2 mutant (D614G) respectively. D614G mutant is the prevalent type in US now. The results indicate that these antibodies have good neutralization activity to wild type and D614G mutant of SARS-COV-2 pseudoviruses, but no cross neutralization activity to SARS and MERS pseudoviruses.
Service highlights
Biacore 8K screening
Screen hundreds of monoclonal antibodies using Biacore 8K to select best antibody pair for use in immunoassay
Multi-gram scale production
We can deliver multi-gram quantity antibodies in a single run
ISO certified facility
Our IVD antibodies manufacturing site is ISO 17025:2017 certified ensuring lot-to-lot consistency