Your Outsourcing Partner for Protein Research and Production Services

To meet the needs of our clients, Exon BioSystems provides protein characterization of our expressed protein to insure the quality of recombinant protein.

Recombinant Protein Expression

Exon BioSystems is a protein contract research and production services company specializing in recombinant protein expression, protein purification, and protein characterization.

Antibody Engineering

Additional areas of expertise include assay development of G-protein coupled receptor, recombinant vaccine development and recombinant antibody production.

Biologics

Exon BioSystems is a protein contract research and production services company specializing in recombinant protein expression, protein purification, and protein characterization.

Products


Catalog #: Size: Concentration: Price:
B706M 10 μg Recombinant Human $320
B706L 50 μg Recombinant Human $955
B706B Bulk Recombinant Human Inquire

Description:

  1. Bone morphogenetic protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is a widely expressed TGFβ superfamily member with important functions during embryogenesis, in the adult, and in disease. Human BMP7 is synthesized with a 29 amino acid (aa) signal sequence, a 263 aa propeptide, and a 139 aa growth factor domain. The growth factor domain of human BMP7 shares 98% aa sequence identity with mouse and rat BMP7. The BMP7 propeptide is cleaved intracellularly but often remains associated with the mature Cterminus. Based on in vivo and in vitro studies, BMP 7 has the potential to be secreted as a disulfidelinked mature homodimer, or particularly as a heteromeric complex that consists of two propeptides oncovalently associated with a mature disulfidelinked homodimer. The presence of the ropeptides in BMP7 appears to stabilize the molecule and provide a docking mechanism for xtracellular storage on molecules such as fibrillin1 and 2. The propeptides themselves do not impart latency to the complex. BMP7 binding to type II receptors rapidly displaces the prodomain:mature molecule interaction and has no effect on activity. But it is suggested that immobilized BMP7 (via prodomain:fibrillin) is inactive, allowing for possible long-term storage of the molecule. BMP 7 interacts with the type 2 receptors Activin RIIA, Activin RIIB, and BMPRII and the type 1 receptors Activin RIA, BMPRIA, and BMPRIB. BMP7 may also be processed into a disulfidelinked heterodimer with either BMP2 or BMP4. Such complexes may show increased potency and range of activity compared to BMP7 homodimers. BMP7 plays a role in a variety of organ systems. It promotes new bone formation and nephron development, inhibits the branching of prostate epithelium, and antagonizes pithelialmesenchymal transition (EMT). In pathological conditions, BMP7 inhibits tumor growth and metastasis, ameliorates fibrotic damage in nephritis, and promotes neuroregeneration following brain ischemia.

Source:

  1. Chinese Hamster Ovary cell (CHO)

Activity:

  1. The ED50 for this effect is typically 0.1-0.5 μg/mL.
  2. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.

Source:

  1. Chinese Hamster Ovary cell (CHO)

Predicted Molecular Mass:

  1. 15.7 kDa (monomer)

Molecular weight on SDS-PAGE

  1. 18-20 kDa, reducing conditions

Activity:

  1. The ED50 for this effect is typically 0.1-0.5 μg/mL.

  2. Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.

Endotoxin Level:

  1. < 1.0 EU per 1 μg of the protein by the LAL method.

Purity:

  1. >95%, by SDS-PAGE under reducing conditions and visualized by silver staining.

Formulation:

  1. Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. See Certificate of Analysis for details.

Technique Documents

  1. Technique Documents