Rabbit monoclonal antibody service

Exonbio offers custom Rabbit Monoclonal Antibody development services using SPIN® (Single Plasma cell INterrogation) Technology. This proprietary technology screens the immune repertoire of rabbits, creating the power to select desired rare antibodies directly from affinity-matured plasma cells.

Benefits

High affinity and specificity

Rabbit monoclonal antibodies consistently demonstrate Kd values that can reach the picomolar level (KD = 10−12M)

Improved immune response to small-size epitopes

Rabbit’s immune system allows them to develop antibodies against small moieties in small molecules, lipids, and polymers, or subtle changes in epitopes such as post-translational modifications or single amino acid substitutions.

Eliminate cross reactive clones

Lower chance of cross-reacting with other proteins as they only detect a single epitope and bind to the target with greater affinity.

More diverse epitope recognition

Lower immunodominance, extensive somatic gene conversion, and hypermutation that characterizes the rabbit immune response result in a more diverse epitope recognition

Benefits

High affinity and specificity

Rabbit monoclonal antibodies consistently demonstrate Kd values that can reach the picomolar level (KD = 10-12 M)

More diverse epitope recognition

Lower immunodominance, extensive somatic gene conversion, and hypermutation that characterizes the rabbit immune response results in a more diverse epitope recognition

Improved immune response to small-size epitopes

Rabbit’s immune system allows them to develop antibodies against small moieties in small molecules, lipids, and polymers, or subtle changes in epitopes such as post-translational modifications or single amino acid substitutions.

Low background noise

Lower chance of cross reacting with other proteins as they only detect a single epitope and bind to the target with greater affinity

Rabbit vs. Mouse Monoclonal Antibodies

Antigen recognition
Affinity
Applications
Mouse
· Limited response to small molecules and peptides
· Narrow spectrum of epitope recognition
· No response to rodent antigens
Nanomolar (10-9M)
Not excellent for IHC, ICC
Rabbit
· High response to small molecules and peptides
· Identifies multiple epitopes per target antigen
Picomolar (10-12M)
Excellent for IHC and ICC
Antigen recognition
Affinity
Applications
Mouse
· Limited response to small molecules and peptides
· Narrow spectrum of epitope recognition
· No response to rodent antigens
Nanomolar (10-9M)
Not excellent for IHC, ICC
Rabbit
· High response to small molecules and peptides
· Identifies multiple epitopes per target antigen
Picomolar (10-12M)
Excellent for IHC and ICC

Timelines

78 Days
Phase 1
Immunization
2 Days
Phase 2
Isolation of plasma cells
7 Days
Phase 3
Cloning and expression
11 Days
Phase 4
Screening and selection

Deliverables

Phase I Timeline Deliverables
Rabbit immunization 2-3 months ELISA data
Phase II Timeline Deliverables
Splenectomy and lymphocyte isolation
First screening with FACS (> a million B cells)
  • Antigen-specific plasma cell identification and isolation.
  • Single plasma cell heavy and light chain amplification.
2 weeks
Supernatant production, and Secondary screening 1 week ELISA data
Phase III Timeline Deliverables
    1. Clone deconvolution
    1. Small scale culture and production, yield ~ 0.5mg Ab per clone
3 weeks Purified Antibody

If you have any technical queries about our products and services, our scientific support team is here to help you.