Description
Protein Name: 2019-nCoV Nucleocapsid Protein (D63G)
Protein Purification: Full-length SARS-CoV-2 Nucleocapsid protein, Met1-Ala419, with point mutation D63G and a C-terminal His-tag. D63G mutation is the same NP mutation which was identified on the SARS-CoV-2 variant (B.1.617) which emerged in India.
Alternative Name: Protein N, NC
Protein Construction: N protein with C-terminal His-tag
Endotoxin: <0.1 EU/ug
Expression Host: E. coli
Protein type: Recombinant
Purification tag: His-tag
Formulation: PBS
Regulation restrictions: For research use only
Shipping: Recombinant proteins are provided as frozen liquid and will be shipped out with dry ice
Stability and storage: The protein is stable in a liquid state at -70℃ for 12 months. Avoid repeated freeze-thaw cycles
Background Information
The nucleocapsid (N) protein is the structural component of the viral structure and it primarily functions to bind coronavirus RNA genome. The N protein is the most abundant protein in coronavirus. It is normally very conserved and is highly immunogenic phosphoprotein. It is also involved in CoV replication cycle and the host cellular response to viral infection. The localization of N to the endoplasmic reticulum (ER)-Golgi region has proposed a function for it in assembly and budding. This protein can be used as a marker for diagnostic tests for Covid-19.
References:
1. https://virologyj.biomedcentral.com/articles/10.1186/s12985-019-1182-0
