SARS-CoV-2 Nucleocapsid Antibodies

Exonbio has developed SARS-CoV-2 Nucleocapsid recombinant monoclonal antibodies for the global IVD and research communities in support of efforts to combat the pandemic. The SARS–CoV–2 nucleocapsid (N) protein is highly homologous to the N protein of SARS–CoV, which is essential for viral RNA replication and packaging into new virions. By using Exonbio’s SPIN® technology platform, highly specific antibodies from plasma cells are generated against nucleocapsid protein of SARS-CoV-2. The recombinant nucleocapsid antibodies developed has no cross reactivity to human coronavirus nucleocapsid protein.

SARS-CoV-2 N-protein IgG mAbs

Clone IDIsotypeSpeciesEC50 (ng/ml)
NP11A2IgG,KRabbit0.57
NP11B6IgG,KRabbit0.60
NP11H9IgG,KRabbit0.84
NP4D2IgG,KRabbit0.95
NP11D9IgG,KRabbit0.96
NP11E9IgG,KRabbit0.97
NP4D8IgG,KRabbit1.02
NP11E4IgG,KRabbit1.17
NP4A6IgG,KRabbit1.37
NP4G8IgG,KRabbit1.55
NP7B1IgG,KRabbit2.33
NP4G6IgG,KRabbit2.35
NP7E2 IgG,KRabbit2.87
NP4H8IgG,KRabbit3.11
NP4B9IgG,KRabbit3.52
NP7C3IgG,KRabbit3.56

Please reach out to us at antibody@exonbio.com for more information about our SARS-CoV-2 Nucleocapsid protein monoclonal antibodies.

Epitope Mapping

High-affinity monoclonal antibodies that fall in different epitope bins allow for antibody pairing for the development of ELISA kit. For detailed Nucleocapsid mAb pairing & clone information contact us.

Figure A: Network plots showing relationship between different monoclonal antibodies by a chord connecting these MAbs.

Validation Data

ELISA: Nucleocapsid protein IgG mAbs are high affinity, with EC50 range between 0.5-3.5 ng/ml.

Figure B: EC50 assay of anti-NP rabbit monoclonal antibodies. Full-length recombinant nucleocapsid protein (Catalog # 19Cov-N150) were coated on ELISA plate at 2 ug/ml.Detection antibody is HRP conjugated goat anti-rabbit IgG antibody at 1:10,000. Data was modeled and analyzed with GraphPad-Prism.

Biacore 3000: Crude supernatants containing rabbit monoclonal anti-NP antibodies were captured on protein A chip and nucleocapsid protein was injected at concentration of 300nM.

Figure C: Affinity screening of anti-NP antibodies on Biacore 3000