Fluorescein Amidite (FAM) rabbit monoclonal antibody
Price range: $250.00 through $450.00
| Product Name | Fluorescein Amidite (FAM) rabbit monoclonal antibody |
| Catalog Number | EX-1G1FMEX-1G1FM |
| Immunogen | FAM conjugated to KLH |
| Host | Rabbit |
| Preparation | Recombinant monoclonal antibody expressed in HEK293F cells and purified by protein A affinity chromatography. |
| Isotype | rabbit IgG, kappa |
| Conjugation | Unconjugated |
| Clonality | Monoclonal |
| Concentration | 1mg/ml |
| Purification | Protein A affinity chromatography |
| Storage buffer | PBS |
| Storage conditions | For long term storage, the antibody should be stored at -20℃ and is stable for 12 months from the date of receipt. Avoid repeated freeze-thaw cycles. For short term storage, keep at 4oC for a week should be fine. |
| Application Dilution | 1:1,000-1:100,000 (platform dependent, customers must test the optimal concentration on their own platform) |
Additional information
| Size | 100µg, 1mg |
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Background information
Carboxyfluorescein (FAM) has become a go-to hapten for oligo-based diagnostics, largely because anti-FAM antibodies bind it with high specificity, giving assays a clean, low-background signal. Its use is especially common in quantitative PCR, where FAM-labeled primers or probes allow real-time monitoring of amplification. That same labeling chemistry carries over well into lateral flow formats: after amplification, FAM-tagged amplicons are captured at the test line by anti-FAM antibody, producing a simple visual result that needs no lab equipment to read. Because FAM shares its core structure with FITC, cross-reactivity between anti-FAM and anti-FITC reagents is common and should be checked for a given kit rather than assumed away. Paired with a second hapten such as biotin in a sandwich format, FAM-based lateral flow assays deliver fast, low-cost molecular detection suited to research, clinical, and point-of-care environments alike.
Datasheet
| Datasheet | Download |
Validation data
| Validation data | Download |
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