Recombinant Influenza A (H1N5) Nucleoprotein



Description Influenza A H5N1 NS2
Influenza A H5N1 (Avian) Nonstructural Protein 2 (NS2)
Synthetic peptide corresponding to 14 amino acids near the center of Avian influenza NS2. Used for blocking the activity of anti-NS2 antibody
Source Recombinant
Format Purified, Liquid Concentration: 0.2 mg/mL (prior to lyophilization).
Concentration 0.2 mg/mL (prior to lyophilization).
Buffer Lyophilized from 10 mM Monosodium Phosphate, 10 mM Disodium Phosphate and 130 mM Sodium Chloride containing 0.1% BSA.
Preservative 0.02% Sodium Azide
Applications Suitable for use in ELISA. Antibody activity is usually completely blocked in ELISA assays by incubating the peptide with equal volume of antibody for 30 minutes at 37°C. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Storage Upon receipt, store (up to one year) at -20°C. Avoid multiple freeze/thaw cycles

Additional information


100µg, 1mg

Background information

Influenza type A (Flu A) viruses are especially prone to reassortment due to their wide host range (humans, dogs, birds, pigs, horses, whales, seals, and other animals). Specifically, the Flu A genome is made up of eight loosely linked segments, each of which harbors at least one important gene. Those genes direct the expression of the major viral proteins such as hemagglutinin (HA) and neuraminidase (NA). In the process of viral reproduction, the linkages between the eight segments of the Flu A genome break apart. Since it is possible for two different Flu A strains to infect a cell simultaneously, some of the genetic segments from one strain can be swapped with another during reproduction. For instance, if a human flu virus and a bird flu virus infect a person, reassortment can intermingle genes from both viruses during replication and create a virus with a protein against which humans have little or no immunity, plus human influenza genes that are more likely to cause sustained human-to-human transmission. In contrast, Influenza B (Flu B) and C viruses do not cause pandemics, most likely due to their limited host range of only humans.


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Validation data

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